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. 2021 Mar 10;22(6):2809. doi: 10.3390/ijms22062809

Figure 5.

Figure 5

Danegaptide negates TGFβ1-evoked changes in the expression of proteins associated with the extracellular matrix. Primary hPTECs were cultured in low glucose (5 mM) ± TGFβ1 (10 ng/mL) ± danegaptide (100 nM) for 48 h. In panel (A), expression of collagen I (Col1), collagen IV (Col4), fibronectin (Fibro) and laminin (panel (B)) was assessed via Western blotting. TGFβ1 upregulated extracellular matrix (ECM) protein expression, an effect reduced by danegaptide (100 nM). Similarly, danegaptide partially reversed TGFβ1-evoked changes in matrix metalloproteinase 3 (MMP3) and integrin-linked kinase 1 (ILK1). Representative blots are shown, with expression normalised by re-probing for ɑ-tubulin as a loading control. Bars correspond to their associated lanes in the respective blot. Results are presented as the mean ± SEM (n = 3; * p < 0.05, *** p < 0.001; one-way ANOVA and Tukey’s post-test).