Figure 5.

Effects of AhR knockdown on EMT, apoptosis, and kidney injury-related markers expression. (A) The protein expression on EMT, apoptosis, and kidney injury-related markers was determined by Western blot after treatment with OTA on HK-2 cells transfected with Control siRNA or siAhR. (B) The mRNA expression on EMT, apoptosis, and kidney injury-related markers was determined by qRT-PCR after treatment with OTA on HK-2 cells transfected with scrambled siRNA (Control siRNA) or AhR-specific siRNA (siAhR). The data are expressed as mean ± S.D. values of three independent experiments with three replicate wells. Different letters indicate significant differences compared with control siRNA at p < 0.05 by Tukey’s studentized range test.