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. 2021 Mar 6;13(3):190. doi: 10.3390/toxins13030190

Figure 6.

Figure 6

Effects of AhR knockdown on Smad2/3 and HIF-1α expression. (A) The protein expression on Smad2/3 and HIF-1α was determined by Western blot after treatment with OTA on HK-2 cells transfected with Control siRNA or siAhR. (B) The mRNA expression on Smad2/3 and HIF-1α was determined by qRT-PCR after treatment with OTA on HK-2 cells transfected with Control siRNA or siAhR. The data are expressed as mean± S.D. values of three independent experiments with three replicate wells. Different letters indicate significant differences compared with control siRNA at p < 0.05 by Tukey’s studentized range test.