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HPLC spectrum of serotonin (a), N-(p-coumaroyl) serotonin (b), and N-feruloyl serotonin (c) in safflower seed extract and standard compounds (A). Toxicity of serotonin (B), N-feruloyl serotonin (C) and N-(p-coumaroyl) serotonin (D) against chondrocytes. The toxicity of serotonin, N-(p-coumaroyl) serotonin, and N-feruloyl serotonin against chondrocytes was measured at concentrations of 0, 10, 50, 100, and 200 μM for 24 h using the LDH assay.
