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. 2021 Mar 2;10(3):374. doi: 10.3390/antiox10030374

Figure 2.

Figure 2

Anti-inflammatory effect of P. spinosa extract on IL-6, IRAK-1, and miR-146a expression levels in LPS (lipopolysaccharide, 1 µg/mL) stimulated young (P3) and older (P15) human umbilical vein endothelial cells (HUVECs) after 6 h of treatment. LPS stimulus downregulated miR-146a (LPS). When treated with P. spinosa extract during LPS stimulation (20/40/80 µg/mL P. spinosa + LPS), cells showed miR-146a upregulation with a decreased expression of both IRAK-1 and IL-6. Results are reported as fold change related to CTRL. Two-tailed paired Student’s t-test: * p < 0.05 LPS vs. P. spinosa + LPS; ** p < 0.01 LPS vs. P. spinosa + LPS; ° p < 0.05 LPS vs. CTRL; °° p < 0.05 LPS vs. CTRL.