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. 2021 Mar 9;14(3):243. doi: 10.3390/ph14030243

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Compounds 1 and 3 affect different steps of virus lifecycle. A549 cells were infected with PR8 and treated or not with furanodienone (1) or curzerene (3) at different phases of the virus life cycle: the compounds were added for 2 h before (PRE); during viral adsorption for 1 h (DUR); immediately after viral adsorption and maintained for 24 h (POST). After 24 h infection, supernatants were recovered and used to infect a fresh monolayer of MDCK cells. The expression of viral Hemagglutinin (HA) was analyzed by ICW assay, using LI-COR Image Studio Software, as described in Methods. The percentage of RFI was calculated in comparison to Infected (I) cells (considered 100%). Values are the mean ± S.D. of three replicates (n = 3) of one out two experiments performed. Statistical significance of the data vs. I was defined as * p < 0.01 and ** p < 0.001.