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. 2021 Mar 12;13(3):916. doi: 10.3390/nu13030916

Figure 1.

Figure 1

Qualitative detection with immunofluorescence staining of CB2 receptor in C2C12 skeletal muscle cells. Confocal image of a representative XY acquisition (60× magnification) of (A) differentiated cells incubated with primary CB2 receptor antibody, (B) differentiated cells incubated with CB2 receptor antibody and its blocking peptide (1/1 ratio), and (C) differentiated cells incubated with secondary antibody alone. Scale bar: 36 μm. Secondary antibody, anti-rabbit Alexa 200 Fluor 568, 1:1000. Images are presented in pseudocolor (LUT = fire) to better show the fluorescent intensity variations in the range 0–2552.