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. 2021 Mar 1;10(3):245. doi: 10.3390/antibiotics10030245

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Characterization of the lytic activity of Psa-his. The lytic activity of Psa (1 U) against C. perfringens HN1314 was determined using the turbidity reduction assay. (a) Optimal pH of Psa-his. The relative activity at pH 6.0 was set as 1. (b) The effect of NaCl on Psa-his lytic activity was determined, and the relative activity at 300 mM NaCl was set as 1. (c) The effects of divalent metal cations on Psa-his lytic activity were determined by the addition of 1 mM MgCl₂, CaCl₂, MnCl₂, ZnCl₂, or EDTA. The relative activities are shown with activity in the absence of divalent cations (with 1 mM EDTA) set as 1. (d) The thermal-stability of Psa-his lytic activity was determined by measuring lytic activity after 10 min of heat treatment at 37, 45, 60, 75, and 100 °C. The relative activity at 37 °C was set as 1.