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. 2021 Mar 1;10(3):245. doi: 10.3390/antibiotics10030245

Figure 5.

Figure 5

Synergistic killing by Psa-his and Psm-his of C. perfringens HN1314 in a checkerboard assay. Serial 2-fold dilutions with PBS (-) of Psa-his and Psm-his (20 μL; individually and in combination) were mixed with 20 μL (4 × 106 cells) of C. perfringens HN1314 on ice. After a 30-min incubation at 37 °C, 2 μL (2 × 105 cells) of each mixture were spotted to GAM/2 agar plates and incubated anaerobically at 37 °C for 12 h. Rows A-F are Psa-his concentrations of 4.3, 8.7, 17.3, 34.6, 69.2, and 138.5 nM. Columns 1-6 are Psm-his concentrations of 1.0, 1.9, 3.9, 7.8, 15.5, and 31 nM.