Table 1.
Lytic spectrum of Psa-his.
| Organism | Strain | Relative Activity (%) |
|---|---|---|
| Gram(+) | ||
| Clostridium perfringens | HN1314 | 100 (3967 U) |
| Clostridium perfringens | st13 | 32 (1289 U) |
| Clostridium perfringens | ATCC13124 | 261 (10,373 U) |
| Clostridium perfringens | SM101 | 25 (997 U) |
| Clostridium perfringens | SM101 (4 h) | 325 (12,911 U) |
| Clostridium perfringens | SM101 (spore) | - |
| Clostridium acetobutylicum | ATCC824 | - |
| Clostridium cellulolyticum | ATCC35319 | - |
| Clostridium coccoides | ATCC29236 | - |
| Clostridium histolyticum | ATCC19401 | - |
| Clostridium lituseburense | ATCC25759 | - |
| Clostridium novyi | ATCC17861 | - |
| Clostridium ramosum | ATCC25582 | - |
| Clostridium sporogenes | ATCC3584 | - |
| Clostridium tetani | KZ1113 | - |
| Clostridioides difficile | ATCC9689 | - |
| Atopobium fossor | ATCC43386 | - |
| Bacillus subtilis | 168 | - |
| Bifidobacterium adolescentis | ATCC15703 | - |
| Enterococcus faecalis | IID682 | - |
| Eubacterium cylindroides | ATCC27805 | - |
| Lactobacillus reuteri | ATCC23272 | - |
| Listeria monocytogenes | HCIPH A5-1 | - |
| Micrococcus luteus | ATCC4698 | - |
| Staphylococcus aureus | ATCC6538P | - |
| Staphylococcus epidermidis | ATCC35984 | - |
| Streptococcus pneumoniae | IID555 | - |
| Streptococcus pyogenes | 124/0207 | - |
| Gram(−) | ||
| Bacteroides fragilis | ATCC25285 | - |
| Escherichia coli | K-12 | - |
| Pseudomonas aeruginosa | PAO1 | - |
| Vibrio cholerae | O1/P1418 | - |
| Vibrio cholerae | O139/MDO-6 | - |
| Vibrio parahaemolyticus | RIMD2210115 | - |
The lytic activities of enzymes were determined by the turbidity reduction assay, using mid-stationary growth-phase cells of various bacteria, and log growth-phase (4 h) cells and spores (spore) of C. perfringens SM101. Spores were prepared by culturing in mDS for 48 h followed by sonication, washing three times with phosphate-buffered saline without Ca and Mg (PBS(-)), and counting using a Thoma cell counter. The culture conditions and media are indicated in Supplementary File Table S1. The cell suspension in PBS(-) (1 OD600) was mixed with Psa (10 μg/mL). The relative activities were obtained by normalizing to that against C. perfringens HN1314 cells, which was defined as 100%. -: No lytic activity was detected.