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. 2021 Mar 15;11(3):822. doi: 10.3390/ani11030822

Table 1.

The primers used for the nested PCR.

Species	Primer Name	Primer Sequence	Orientation	The Run of Nested PCR
S. bovifelis	SF1 ¹	ATGGCGTACAACAATCATAAAGAA	Forward	First
	SkatR	CAGGCTGAACAGHABTACGA	Reverse	First
	V2bo3	ATATTTACCGGTGCCGTACTTATGTT	Forward	Second
	V2bo4	GCCACATCATTGGTGCTTAGTCT	Reverse	Second
S. cruzi	SF1 ¹	ATGGCGTACAACAATCATAAAGAA	Forward	First
	SsunR2	GTGCCTCCCAGGCTGAAYAG	Reverse	First
	GsScruF	TGTATCTACTTACGGCAGGTATCTTT	Forward	Second
	GsScruR	CGTAGTTAGATCCATATCACTCGGTA	Reverse	Second
S. hirsuta	SF1 ¹	ATGGCGTACAACAATCATAAAGAA	Forward	First
	SkatR	CAGGCTGAACAGHABTACGA	Reverse	First
	GaHiEF ²	GTTGTGCGGTATGAATTATCAACCT	Forward	Second
	GaHiER ²	GGTAAGAACTGGAATGGTTAATATCAG	Reverse	Second
S. hominis	VohoF	GTGCGGTATGAACTGTCTACTGCT	Forward	First
	VohoR	AATACCTGCCCGGCCTTAAC	Reverse	First
	GaHoEF ²	TCTCTGGTTTTGGTAACTACTTCGT	Forward	Second
	GaHoER ²	CAGACACTGGGATATAATACCGAAC	Reverse	Second

¹ [24], ² [19].