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DSF or DSF/Cu2+ inhibits spheroid formation in the head and neck squamous cell carcinoma (HNSCC) cell lines. (A) Cells were exposed to DSF or DSF/Cu2+ for 72 h, and representative images are shown (×50 magnification). (B) Histogram shows the statistical analysis of spheroid numbers. ** p < 0.01 one-way ANOVA compared to control.
