Table 2.
Mean diameter (size, nm) and polydispersity index (PDI) of freshly prepared ATRA-TPGS formulations reconstituted from lyophilized powder. The zeta potential (ƺ) values were detected on fresh ATRA-TPGS micelles in water and in the HEPES buffer. Each value represents the mean ± SD (degrees of freedom = 3).
| ATRA:TPGS (w/w) | Size (nm) Fresh |
PDI Fresh |
Size (nm) Lyophilized |
PDI Lyophilized |
ƺ (mV) mQ Water |
ƺ (mV) HEPES |
|---|---|---|---|---|---|---|
| 1:20 | 13.8 ± 0.1 | 0.24 ± 0.03 | 15.0 ± 0.6 | 0.32 ± 0.09 | −7.2 ± 0.5 * | 0 ± 3 * |
| 1:30 | 17 ± 5 * | 0.3 ± 0.1 * | 19 ± 5 | 0.31 ± 0.02 | −9 ± 3 | 5 ± 1 |
| 1:40 | 12.6 ± 0.4 | 0.17 ± 0.06 | 14.1 ± 0.8 | 0.2 ± 0.1 | −13 ± 2 * | 0 ± 2 * |
| 1:50 | 11.9 ± 0.4 | 0.13 ± 0.06 | 14.7 ± 0.1 | 0.212 ± 0.001 | −11 ± 1 | 5 ± 1 |
| 1:60 | 11.8 ± 0.4 | 0.14 ± 0.03 | 21 ± 7 * | 0.4 ± 0.1 * | −13 ± 3 * | 4 ± 2 |
| 1:70 | 11.4 ± 0.1 | 0.11 ± 0.01 | 14.4 ± 0.2 | 0.19 ± 0.03 | −10.1 ± 0.7 | 4 ± 1 |
* indicates statistical difference vs. sample mean (p < 0.05).