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. 2021 Mar 12;13(3):377. doi: 10.3390/pharmaceutics13030377

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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In vitro assessment of (top images) the cytotoxicity (normal phase mode) and (bottom images) transfection efficiency (fluorescent mode) of pLbL formulations using microscopy. Images taken 24 h after transfection of 90 ng eq. of eGFP mRNA, formulated either in liposomes or in LP using pLbL strategy (ratio mRNA/LAH4-L1 = 1:20 w/w) on DC 2.4 ((A), (a–h)) and HeLa ((B), (a’–h’)) cells. Lipofectamine 2000^TM was used as positive control. Scale bar: 100µm. (a,a’) Cells alone (b,b’) LAH4-L1 peptide (c,c’) Liposomes, (d,d’) LP (e,e’) Naked mRNA (f,f’) Lipofectamine 2000^TM (g,g’) pLbL Liposomes mRNA (h,h’) pLbL LP mRNA.