Figure 6.
In vitro evaluation at +24 h (top) of transfection efficiency through the measurement of luminescence intensity (Bright-Glo luciferase assay) and (bottom) cell viability (PrestoBlue assay) obtained after transfection of 90 ng eq. of either Fluc mRNA or luciferase-pcDNA3.1, formulated in LP using pLbL strategy (ratio nucleic acids/LAH4-L1 = 1:20 w/w) on HeLa (A,C) and DC 2.4 (B,D) cells. Lipofectamine 2000TM was used as positive control. Data are presented as mean ± SD and statistically analyzed using one-way ANOVA followed by Tukey’s multiple comparison test (ns: p > 0.01, **: p <0.001, ***: p < 0.0001 and ****: p < 0.00001).