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. 2021 Mar 10;13(3):199. doi: 10.3390/toxins13030199

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The effects of ZEA on the MAPK family proteins and PI3K-AKT-mTOR pathway. (a) Heat map of changes in expression levels of selected genes 24 h after ZEA treatment (20μM). (b) Functional enrichment pathways of chicken granulosa cells treated with ZEA. (c,d) Western blot determined the expression of p-PI3K, PI3K, p-AKT, AKT, p-mTOR, and mTOR. (e,f) The expression of MAPK family proteins detected by Western blot. The results are presented as mean ± SD. * p < 0.05, ** p < 0.01 versus the control group.