Figure 3.

(a) In vitro planktonic MSSA susceptibility to free and liposomal RFB assessment through broth microdilution method, followed by turbidity measurement (OD_{570 nm}), RFB concentrations tested ranged from 0.002 to 0.200 µg/mL. The negative control corresponds to planktonic MSSA in MHB (CTR). (b) Determination of viable bacterial cells (MTT assay) and (c) biofilm biomass quantification (CV method) after mature MSSA biofilm incubation with a RFB concentration ranging from 0.001 to 10.000 µg/mL. Statistical comparisons were determined by two-way ANOVA (Tukey’s multiple comparisons test) analysis of variance compared between formulation groups. Results are expressed as mean ± SD of at least three independent experiments.