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. 2021 Mar 15;10(3):457. doi: 10.3390/antiox10030457

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of 13R,20-diHDHA-induced ROS generation on mammospheres formation. (A,B) The effect of 13R,20-diHDHA (20 μM) on ROS generation in MDA-MB-231 and MCF-7 cells was determined using DCF-DA staining. Images were obtained by FACS and fluorescence microscopy at 4x magnification and representative photos are shown (scale bar: 100 μm). (C) Mammospheres were pretreated with/without NAC (10 mM) for 1 h prior to treatment with 20 μM 13R,20-diHDHA. After 7 days, mammospheres formation was determined. Representative images of colonies were recorded. The data from triplicate experiments are presented as the mean ± SD. * p < 0.05 versus the DMSO-treated control group.

Effect of 13R,20-diHDHA-induced ROS generation on mammospheres formation. (A,B) The effect of 13R,20-diHDHA (20 μM) on ROS generation in MDA-MB-231 and MCF-7 cells was determined using DCF-DA staining. Images were obtained by FACS and fluorescence microscopy at 4x magnification and representative photos are shown (scale bar: 100 μm). (C) Mammospheres were pretreated with/without NAC (10 mM) for 1 h prior to treatment with 20 μM 13R,20-diHDHA. After 7 days, mammospheres formation was determined. Representative images of colonies were recorded. The data from triplicate experiments are presented as the mean ± SD. * p < 0.05 versus the DMSO-treated control group.