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. 2021 Mar 2;10(3):282. doi: 10.3390/pathogens10030282

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Evaluation of PvMSP8 protein production. (A) PvMSP8 recombinant protein production. Recombinant protein was affinity-purified from culture supernatants with nickel affinity chromatography. SDS-PAGE (Coomassie Blue staining) and Western blot (using anti-His6 monoclonal antibody) analysis demonstrated proteins of the expected size (54 kDa). (B) Evaluation of IgG responses against PvMSP8 and PvMSP10 recombinant protein by ELISA. Total IgG levels of plasma samples from confirmed P. vivax positive individuals in comparison with healthy individuals. (C) Cross-reactivity assays for the evaluation of PvMSP8 specific IgG antibodies responses by ELISA. Total IgG levels in plasma samples from P. falciparum-infected Senegalese subjects (Pf Senegal), P. falciparum-infected individuals from the Peruvian Amazon (Pf Peru), P. vivax-infected individuals from the Peruvian Amazon (Pv Peru), and healthy individuals against PvMSP8 were compared. The horizontal line for each group shows the median. The statistical significance of a difference between groups is indicated with upper horizontal branches. * p < 0.05, *** p < 0.001; **** p < 0.0001, ns no significant.