Figure 1.

Map of vectors expressing SARS-CoV-2-based fusion proteins. Shown are the map of pVAX1 vector as well as the cloning strategies pursued to obtain both pVAX1-Nef^mut and pVAX1-Nef^mutfusion vectors. Restriction sites where the SARS-CoV-2 ORFs were inserted are highlighted in the red box. On the bottom right, shown are both amino acid and nucleotide sequences of Nef^mut C-terminus in the pVAX1-Nef^mutfusion vector (in italics), as well as of the GPGP linker overlapping the Apa I restriction site (underlined sequence).