Figure 6.

SARS-CoV-2-specific CD8⁺ T cell immune responses induced in spleens from mice immunized by combination of Nef^mut-based DNA vectors. CD8⁺ T cell immune response in C57 Bl/6 mice inoculated i.m. with either the DNA vector expressing Nef^mut alone (3 mice) or the indicated combinations of vectors expressing Nef^mut fused with the SARS-CoV-2 antigens (5 mice per group). The CD8⁺ T cell immune responses were measured using the indicated peptides and combination thereof. At the time of sacrifice, 2.5 × 10⁵ splenocytes were incubated overnight (ON) with or without 5 μg/mL of either unrelated or SARS-CoV-2-specific peptides in triplicate IFN-γ EliSpot microwells. Shown are the numbers of SFU/well calculated as mean values of triplicates after subtraction of mean values measured in wells of splenocytes treated with unspecific peptides. Reported are intragroup mean values + standard deviations also. Circles sign values measured in cultures of splenocytes isolated from each injected mouse The results are representative of two independent experiments.