. 2021 Mar 10;13(3):449. doi: 10.3390/v13030449

Table 1.

Details of human endogenous retrovirus (HERV) primers and genomic regions targeted.

Target	Amplicon Size	Primers	Sequence (5′–3′)	Target Region or Reference ¹	Predicted Targets ²
HERV-K Gag	113 bp	Fwd	TTCCCGAGTACGTCTACAGTGA	[19]	1
HERV-K Gag	113 bp	Rev	GGTGTTTCTCATCAGGTGGAAT	[19]	1
HERV-K Np9	113 bp	Fwd	GTTAGTCTACAGGTGTATCCA	GenBank Y17832.2 8836–8948	1
HERV-K Np9	113 bp	Rev	CTGTCTCTTTTCCCTACATTTCC	GenBank Y17832.2 8836–8948	1
HERV-W Gag	81 bp	Fwd	TCAGGTCAACAATAGGATGACAACA	[21]	1
HERV-W Gag	81 bp	Rev	CAATGAGGGTCTACACTGGGAACT	[21]	1
HERV-E Pol	98 bp	Fwd	CTCTACACAGTTAGGCTCG	Chr13 40,349,095–40,349,192	1
HERV-E Pol	98 bp	Rev	GTGAAAATCCCCGCATAACC	Chr13 40,349,095–40,349,192	1
HERV_E Env	168 bp	Fwd	ATTTGATGCTTGTGCAGCCA	[22]	2 ³
HERV_E Env	168 bp	Rev	TTCTTTTTCCAAGTAGCCCAAAT	[22]	2 ³
HERV_H Pol	118 bp	Fwd	GCCAAACACATATACTCTCC	Chr8 8,066,673–8,066,790	1
HERV_H Pol	118 bp	Rev	GAAGAGTGACTGGGATGAAG	Chr8 8,066,673–8,066,790	1

¹ Chromosome position based on Human Genome Sequence version hg18. ² Number of amplified targets predicted within the genome by MFEPrimer analysis. ³ Two DNA targets are on chr17 and chr19, which are both 168 base pairs in length and share 95% nucleotide sequence identity.