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. 2021 Mar 7;9(3):266. doi: 10.3390/biomedicines9030266

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Semiquantitative analysis of CD3+, CD4+, CD8+, FoxP3+ cells and mast cells. Semi-quantitative analysis of antibodies against CD3+, CD4+, CD8+, FoxP3+ cells and mast cells (Giemsa staining), in the different follicular compartments of interest and their corresponding perifollicular mesenchymal compartments, representing the connective tissue sheath around each follicular compartment; infundibulum, bulge region, proximal area, and bulb. For mast cells, the y-axis shows the mean number of mast cells per follicular and perifollicular compartment. For CD3+, CD4+, CD8+ and FoxP3+ expression was scored as follows: 0, no expression; 1, mean cell count per field (MCCPF) between 1 and 10; 2, MCCPF between 10 and 20; or 3, MCCPF more than 20. H (n = 3), LPP (n = 11), FFA (n = 8). LPP, lichen planopilaris; FFA, frontal fibrosing alopecia.