Figure 2.

CIBERSORTx analysis with scRNA-seq reference of nine major cell types. (A) t-SNE projection of scRNA-seq data from 21 HNSCC samples colored by nine major cell clusters. (B) Heatmap of the relative cell fractions of the nine major cell types for each sample estimated by CIBERSORTx. The percentage is normalized by the corresponding mean within each cell type. The tissue origin and tumor stage are annotated as side bars. (HP = Hypopharynx; L = Larynx; OC = Oral Cavity; OP = Oropharynx). (C) Association between cell proportions and overall survival in patients with HNSCC profiled by TCGA. Estimated cell proportions were stratified by a half–half split, and the separation between survival curves was evaluated using a log-rank test.