Table 1.
Nucleotide sequence and position of newly acquired ectopic spacers in CRISPR3 of the BIMs obtained after a challenge with phage 53.
| BIM | Sequence of New Spacer (5′-3′) | Position in CRISPR3 | Protospacer Position in Phage 53 Genome | PAM NGGNG |
|---|---|---|---|---|
| BIM1 | TGATAGTAAAATATTGTCATCATTGAATAC | 6 | None * | None |
| CATTACAGACACAGGAGAAGGCGGCTATTA | 4 | 22837-22866, orf22 | TGGTG | |
| BIM2 | TTATGCAAACGGTGGCCTAGTCCACAAGAA | 4 | 14360-14389, orf14 | CGGCG |
| BIM3 | AGTTGATGGTAAAACGGTGGAATGACCATA | 4 | 31076-31105, orf34 | TGGCG |
| BIM4 | AAACGTCAAAAAAGCTGGTAGTAAGGTCAA | 4 | 11789-11818, orf14 | TGGCG |
| BIM5 | TGTTCAGTATCGTCGACTTCATTCCCCAAA | 4 | 10537-10508, orf14 | CGGCG |
| BIM6 | TGTTCAGTATCGTCGACTTCATTCCCCAAA | 4 | 10537-10508, orf14 | CGGCG |
* Did not match any region in phage 53 genome and no significant results were obtained from BLAST analysis.