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. 2021 Mar 13;22(6):2913. doi: 10.3390/ijms22062913

Figure 1.

Figure 1

The regulatory network of 22 nucleotides (nt)-long miRNAs and NBS-LRR mRNAs involved in production of phasiRNAs. The 22-nt miRNA guides AGO protein to cleave the target site on the NBS-LRR transcript, triggering dsRNA synthesis mediated by RDR6 (RNA-DEPENDENT RNA POLYMERASES 6) and SGS3 (SUPPRESSOR OF GENE SILENCING 3). dsRNA is subsequently processed by DCL4 (DICER-LIKE 4) and DRB4 (DOUBLE-STRANDED-RNA-BINDING PROTEIN 4) to generate a cluster of 21-nt phased siRNAs (phasiRNAs). These 21-nt phasiRNAs are loaded into AGO proteins, which in turn can lead to NBS-LRR mRNAs cleavage. On the other hand, these siRNAs will depress more new targets.