Table 2.

Univariate analysis and biomarker identification studies (ROC-AUC) of candidate metabolites in plasma collected on Day-0 (N = 16) and Day-7 (N = 17) from Holstein recipients transferred with vitrified/warmed embryos and diagnosed at pregnancy endpoints Day 40, Day 62, and birth.

	Day	Pregnancy	Univariate Study			Biomarker Discovery Study
Metabolite	Plasma	Endpoint	p	Open	Pregnant	p	FCh	ROC-AUC	Open	Pregnant
Hydrocinnamic acid	0	Birth	0.059	362 ± 34	255 ± 44	0.044	0.495	0.797	6/8	6/8
Hydrocinnamic acid	0	Day-40	0.012	400 ± 34	254 ± 26	0.005	0.666	0.900	5/6	8/10
2-hydroxybiphenyl	0	Day-40	0.013	271 ± 30	363 ± 23	0.031	−0.395	0.900	5/6	8/10
Glycerol-phosphate	0	Day-40	0.017	1315 ± 307	1401 ± 238	0.062	−0.586	0.883	6/6	8/10
Alanine	7	Birth	0.065	1662 ± 182	2163 ± 172	0.065	−0.357	0.792	6/8	6/9
Lysine	7	Birth	0.067	148 ± 25	223 ± 24	0.049	−0.497	0.778	6/8	7/9
Threonine	7	Day-62	0.051	93 ± 18	141 ± 15	0.052	−0.669	0.800	5/7	8/10

Open and pregnant: concentrations in arbitrary units (Univariate study) and samples correctly identified/total samples (Biomarker discovery study). p values: Kruskal-Wallis test or General Linear Model procedure with Bonferroni correction p < 0.05 (Univariate study) and t-test (Biomarker discovery study). Significant differences: p < 0.05. Tendency (bold) 0.05 < p <0.07. FCh = Log Fold change. ROC-AUC: Receiver Operator Characteristic—area under curve.