Table 3.

Univariate and biomarker discovery study (ROC-AUC) of candidate metabolites at pregnancy endpoints (Day 40, Day 62, and birth) in Day-0 (N = 13) and Day-7 (N = 13) plasma of Asturiana de los Valles recipients transferred with vitrified/warmed embryos (data from Day-62 and Day-40 were the same).

	Day	Pregnancy	Univariate Study			Biomarker Discovery Study
	Plasma	Endpoint	p	Open	Pregnant	p	FCh	ROC-AUC	Open	Pregnant
Stearic acid	0	Birth	0.022	3668 ± 96	3204 ± 104	0.007	0.191	0.881	6/7	6/6
Palmitic acid	0	Birth	0.007	2901 ± 215	2136 ± 221	0.013	0.367	0.976	6/7	6/6
6-methyl-5-hepten-2-one	0	Birth	0.055	108 ± 1.9	115 ± 2.0	0.055	−0.078	0.833	6/7	5/6
Palmitic acid	0	D40–D62	0.015	286 ± 17	223 ± 16	0.020	0.342	0.929	5/6	6/7
Hippuric acid	7	Birth	0.021	2385 ± 144	1663 ± 148	0.071	0.430	0.857	5/7	5/6
Hippuric acid	7	D40–D62	0.045	2458 ± 230	1839 ± 213	0.045	0.454	0.833	4/6	6/7
L-Valine	7	D40–D62	0.056	4254 ± 443	3607 ± 410	0.056	0.596	0.857	5/6	6/7
N-(2-hydroxyethyl) iminodiacetic acid 2	7	D40–D62	0.022	10,743 ± 492	9150 ± 455	0.037	0.230	0.881	5/6	7/7
Dehydroascorbic acid	7	D40–D62	0.034	1213 ± 66	1012 ± 49	0.034	0.302	0.857	5/6	6/7

Open and pregnant: concentrations in arbitrary units (Univariate study) and samples correctly identified/total samples (Biomarker discovery study). p values: Kruskal-Wallis test or GLM procedure with Bonferroni correction p < 0.05 (Univariate study) and t-test (Biomarker discovery study) significant differences: p < 0.05. Tendency (bold): 0.05 < p < 0.072. FCh = Log Fold change.