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. 2021 Mar 5;7(3):187. doi: 10.3390/jof7030187

Table 2.

Details of primers and annealing temperatures used for the amplification of gene targets in this study.

Region Primer Orientation Annealing
Temperature (°C)
Reference
ITS * ITS5 Forward 50 [29]
ITS4 Reverse 50 [29]
rpb2 fRPB2-5F Forward 54 [30]
fRPB2-7cr Reverse 54 [30]
tef1-α Tef1-728F Forward 52 [31]
Tef1-986R Reverse 52 [32]

* ITS = internal transcribed spacer; rpb2 = RNA polymerase II; tef1-α = translation elongation factor 1-α.