Table 2.
Details of primers and annealing temperatures used for the amplification of gene targets in this study.
Region | Primer | Orientation | Annealing Temperature (°C) |
Reference |
---|---|---|---|---|
ITS * | ITS5 | Forward | 50 | [29] |
ITS4 | Reverse | 50 | [29] | |
rpb2 | fRPB2-5F | Forward | 54 | [30] |
fRPB2-7cr | Reverse | 54 | [30] | |
tef1-α | Tef1-728F | Forward | 52 | [31] |
Tef1-986R | Reverse | 52 | [32] |
* ITS = internal transcribed spacer; rpb2 = RNA polymerase II; tef1-α = translation elongation factor 1-α.