Fig. 4.

Simulations of iron deficiency. (A) Simulated growth rates and exchange fluxes with various θ values for 50% iron uptake compared with the reference. (B) Experimentally measured differential expression compared with simulations upon 50% reduction in iron uptake with various θ values. The color of the enzyme name represents measured differential expression upon iron deficiency (i.e., blue means measured down-regulation and red up-regulation). Heatmap represents simulated differential expression based on predicted protein abundances. Complex abbreviations: QCR, ubiquinol cytochrome-c reductase; SDH, succinate dehydrogenase. (C) Iron usage of Erg25 and Ole1 with various θ values. (D) Sensitivity analysis of iron-containing proteins with 50% iron uptake and θ value of 0.5. (E) Reduced cost analysis of amino acid uptake for growth under unlimited and 50% iron uptake conditions with θ value of 0.5. Color represents the reduced cost value. A higher value indicates a greater impact of an increase in the amino acid uptake on growth rate.