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. 2021 Mar 14;14(3):260. doi: 10.3390/ph14030260

Figure 4.

Figure 4

Synergistically inhibitory effect of TSAIII and Cyclo on migration and invasion of human osteosarcoma cells. (A) Human 143-B and HOS osteosarcoma cells were treated with various concentrations of TSAIII (0 and 4 μM) and/or Cyclo (0 and 50 μM) and then harvested to detect the expression and activation of cytoskeletal-related proteins through immunoblotting. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as the internal control. (B) A change in the expression of cytoskeletal F-actin was observed in human 143-B and HOS osteosarcoma cells using immunofluorescence analysis. (C) The migration and invasion capacities of human 143-B and HOS osteosarcoma cells were measured after treatment with TSAIII in the presence or absence of Cyclo for 18 h (migration) or 24 h (invasion). ** p < 0.01 versus control; # p < 0.05 versus treatment with Cyclo alone. Cyclo denotes Cyclo(RGDyK) (Intergin inhibitor). Scale bar: 50 μm.