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. 2021 Mar 18;118(12):e2011876118. doi: 10.1073/pnas.2011876118

Fig. 3.

Fig. 3.

Divergent neuronal cell cycle response to Aβ. (A and B) Image stack from 45-h recording of representative mAG-hGem (1-110)-transduced cultured hippocampal neurons responding to Aβ exposure by either (A) continuingly increasing mAG fluorescence intensity (mAG+; green) without DRAQ7 uptake (D7−), or (B) transient mAG fluorescence intensity followed by DRAQ7 uptake (D7+; red). (Scale bar, 25 μm.) (C) Time course of mAG fluorescence intensity of mAG+/D7− (green) and mAG+/D7+ (red) neuronal populations upon 0.5 μM oAβ exposure (n = 30 to 37; P < 0.0001; linear regression analysis). Transient mAG fluorescence trace of vehicle-treated neurons (black) is provided for comparison (n = 20 ± 5). Means ± SEM are presented by the center trace and broken lines, respectively. (D) Comparison of mean linear regression slope values of mAG fluorescence intensity traces shown in C (n = 30 to 37; ****P < 0.0001; Student’s t test). (E) Area cell count comparison between mAG+/D7− and mAG+ D7+ oAβ-treated neurons (n = 8; ns, not significant; Student’s t test). (F) mAG fluorescence levels at 3 h after oAβ challenge in neurons classified as mAG+/D7− or mAG+/D7+ after 45 h of live imaging (n = 14 to 24; ***P = 0.0008; Student’s t test).