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. 2021 Mar 15;118(12):e2025196118. doi: 10.1073/pnas.2025196118

Fig. 1.

Fig. 1.

Deletion of Brg1 using Eya1CreER or Sox2CreER in otic placode results in lack of neurosensory cell fate specification. Sections are transverse and dorsal is up. For whole-mount images, anterior is up. (A) Anti-Brg1 immunostaining on sections showing Brg1 expression in otic placode (Op) and otocyst (Ot). The Left is a merge of red and Hoechst nuclear staining. (Scale bars, 50 μm.) (B and G) H&E-stained sections of otocyst of Eya1CreER and Brg1cKO/cKO (Eya1CreER;Brg1fl/fl) or Sox2CreER and Brg1cKO/cKO (Sox2CreER;Brg1fl/fl). (Scale bars, 50 μm.) (C, D, H, and I) ISH on sections for Sox2 or Dlx5. (Scale bars, 50 μm.) (E, F, and J) Whole-mount ISH for Neurog1 (dorsal view, E and J) or Neurod1 (lateral view, F) in otic and cranial ganglia. (Scale bars, 120 μm.) (K) Schedules for tamoxifen administration. n = 6 embryos for each genotype. ED, endolymphatic duct; V to X, Vth to Xth cranial ganglion.