Figure 1.

Multiple sequence alignment of the γ-CA sequences performed by PROMALS3D alignment program. Abbreviations: Methanosarcina thermophile—Cam; Pyrococcus horikoshii—Cap; Thermosynechococcus elongatus BP-1—CcmM; Esherischia coli—EcoCA-γ; Pseudomonas aeruginosa—PA5540; Persephonella hydrogeniphila—γ-PhCA; Persephonella marina—γ-PmCA; Thermus thermophilus—γ-TtCA; and Thermosulfidibacter takaii—γ-TtkCA. Residues are colored by the extent of conservation. Residues in the red boxes are important for catalysis while Zn²⁺ coordinating His residues are shown in green boxes. The black box depicts the insert present for Cam, CcmM, and EcoCA-γ. The blue dots show proton shuttling Glu residues identified in Cam. Yellow box depicts residues around the hydrophobic region in the catalytic site of γ-PmCA.