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(A,B) show position of His69 in the active sites of γ-PhCA and γ-PmCA respectively as well as plots illustrating its bond distance from Zn2+ during simulations at 300 K, 363 K, 393 K and 423 K. (C) shows His70 taking up the fourth coordination position in EcoCA-γ. (D,E) show the active site of γ-TtCA and γ-TtkCA, with Pro68 and Thr66 respectively, in place of γ-PhCA’s His69.
