Figure 8.

Summary of gentamicin-induced perturbations in various metabolic pathways in the kidney. Schematic of significantly enriched pathways in the kidney metabolism of rats based on genes significantly altered at 7 h after gentamicin exposure. We propose that gentamicin exposure leads to endosomal membrane disruption and observed downregulation of several lysosomal genes and genes involved in sphingolipid metabolism, as well as upregulation of genes involved in glycerophospholipid metabolism. Subsequent release of gentamicin into the cytosol leads to downregulation of genes involved in the TCA cycle and amino-acid metabolism, which in turn induces mitochondrial damage and impaired energy metabolism. Arrows indicate the reaction directionality, with the color indicating the genes responsible for them either being significantly (FDR < 0.1) upregulated (red), downregulated (green), or unchanged (black). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)