Figure 3.

Platelet-derived products differentially modulated angiogenesis in a 3D fibrin gel. (A) 3D HUVEC cultures were treated with either PRP, PRP-HA or PL for 4 days. Representative images showing massive enhancement of angiogenesis (20% PRP, 20% PRP-HA) or slight endothelial proliferation from the EC coated beads (20% PL) compared to control conditions (control and heparin) at day 4. Scale bar: 150 µm. (B) Quantification of morphometrical parameters of the capillary network was performed by a computerized method on images taken on day 4. Representative parameters measured were total length, total branch length, number of extremities and number of anchorage junctions per sphere. Graphs are representative of three independent experiments. One hundred spheres were quantified for each experimental condition and compared to the heparin-treated cultures. Significant from heparin as measured by the one-way ANOVA followed by Dunett’s multiple comparison test (* p < 0.05, ** p < 0.01, *** p < 0.005, **** p < 0.001).