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. 2021 Mar 15;9(3):261. doi: 10.3390/vaccines9030261

Figure 5.

Figure 5

Identification of the protein antigens recognized by IgG in serum from mice colonised with wild type and double mutant S. pneumoniae strains. Antigen specific IgG binding data obtained by probing a protein array containing 254 S. pneumoniae protein antigens with sera from mice colonised twice with the 6B, ∆cps/psaA, or ∆cps/proABC strains. (A) Heatmap of mean IgG binding levels to the top 16 proteins recognised by IgG in colonised mouse sera (n = 5 mice). (B) Aggregated MFI for IgG binding to the 30 antigens with the highest level of IgG binding in serum from mice colonised twice with the 6B, ∆cps/psaA, or ∆cps/proABC strains, or sham colonized with PBS. Each symbol represents data from 1 mouse, bars represent mean and error bars SD. Asterisks represent statistical significance compared to the uncolonised group (Kruskal-Wallis with Dunn’s multiple comparisons posthoc test; ***, p < 0.001). (C) Binding results in sera from mice colonised twice with the 6B strain, ∆cps/psaA, or ∆cps/proABC strains, or sham colonised to the 6 antigens with the highest level of IgG binding in serum obtained from wild type colonized mice. Each symbol represents data from 1 mouse, bars represent mean and error bars SD. Asterisks represent statistical significance compared to the uncolonised group (two-way ANOVA with Dunnett’s for multiple comparisons, **, p < 0.01; ***, p < 0.001, ****, p < 0.0001).