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. 2021 Mar 15;22(6):2959. doi: 10.3390/ijms22062959

Figure 1.

Figure 1

(A) Expression of Grainyhead-like 3 (GRHL3) transcript was readily detectable in freshly prepared normal human urothelial (NHU) epithelium and cultured primary urothelial epithelial cells (M × 3 and M × 8). (B–D) GRHL3 expression was downregulated in invasive bladder cancer cell lines. (B) RT-qPCR and (C) semi-quantitative RT-PCR show lower expression of GRHL3 transcript in RT112 compared to non-invasive RT4 cells and absence of GRHL3 transcript in anaplastic T24 cells. *** indicates p-value < 0.001 (D) GRHL3 protein expression was only detected in RT4 cells by Western blot.