Figure 3.

GRHL3 overexpression reduces migration in scratch wound assay. (A) Representative scratch wound experiment, displaying the original scratch wound gap in each picture by red lines during gap closure over 12 h. (B) Quantification of the “wound” closure shows that ectopic GRHL3-expressing T24 cells migrate significantly more slowly compared to empty vector controls (T24 + pLX304) at 6, 9 and 12 h (p = 0.0013 at 9 h). Seven replicates were performed in this experiment. ** indicates p-value < 0.01.