Skip to main content
. 2021 Mar 15;22(6):2959. doi: 10.3390/ijms22062959

Figure 4.

Figure 4

Ectopic overexpression of GRHL3 impairs invasion capacity of T24 cells in Boyden chamber assay. (A) Representative images from Boyden chamber assays showing “invaded” T24 cells stained with crystal violet, indicating lower invasive potential in GRHL3-overexpressing cells. Scale bar, 200 µm. (B) Analysis of the cell-covered area using software demonstrated that GRHL3-overexpressing cells (T24 + GRHL3) invaded through membranes to a significantly lower extent than empty vector controls (T24 + pLX304; p = 0.0317). SD bars are shown for a total of 70 images analyzed. * indicates p-value < 0.05.