Table 3.
Antibodies used for Western blots.
| Scheme | Antigen | Clone | Dilution | Provider |
|---|---|---|---|---|
| Mouse | GRHL3 | C-12, sc-398838, IgG2b | 1:1000 | Santa Cruz, Dallas, TX, USA |
| Mouse | GRHL3 | Aa529-578 | 1:500 | Lifespan Biosciences, Seattle, WA, USA |
| Horse | Mouse IgG | HRP-linked, #7076 |
1:2000 | Cell Signaling Technology, Danvers, MA, USA |
| Rabbit | Total Akt | #75692S (D9R8K) | 1:2000 | Cell Signaling Technology, Danvers, MA, USA |
| Rabbit | Phospho-Akt (Ser473) | #4060 (D9E) | 1:2000 | Cell Signaling Technology, Danvers, MA, USA |
| Rabbit | PTEN | #9559 (138G6) | 1:1000 | Cell Signaling Technology, Danvers, MA, USA |
| Chicken | Rabbit IgG | HRP-linked, sc-2955 |
1:2000 | Santa Cruz, Dallas, TX, USA |
| Mouse | β-Actin | AC-15 | 1:10,000 | Sigma-Aldrich, St. Louis, MO, USA |