Figure 3.

Arabidopsis LPEAT2 does not colocalize with the autophagosomal marker ATG8 in cells in the absence or presence of autophagy inducer BTH. BY-2 cells were transiently-cotransformed (as indicated by labels) with mGFP-LPEAT2 (green) and RFP-ATG8a (red) and incubated with or without 10 µM BTH. Cells were subsequently formaldehyde-fixed and processed for CLSM. Shown are representative images, as well as the corresponding merged images for each set of cotransformed cells. Boxes represent the portion of the cell co-expressing mGFP-LPEAT2 and RFP-ATG8a and incubated with BTH shown at higher magnification in the panels below. Note an increase in the number of RFP-ATG8-labelled puncta upon induction of autophagy with BTH indicating formation of autophagosomes. Note also a lack of colocalization between mGFP-LPEAT2 and RFP-ATG8a. Scale bar, 10 μm.