Figure 8.

Relative amount of different form of ATG8 proteins in total protein extracts from leaves (0 DAF) of wild-type Arabidopsis plants (Col-0) and LPEAT1 and LPEAT2 overexpressors, lpeat1 and lpeat2 single mutants and lpeat1 lpeat2 double mutant grown in soil under standard conditions. (A) Selected western blot analysis on urea gel; equal amounts of protein (10 µg) were loaded and separated on the urea gel. The blots were incubated with polyclonal antibodies (anti-APG8a/ATG8a (abcam)) against ATG8 protein. (B) Bands intensity determined by “Image Lab 6.01” program. Means ± SD are presented (n = 3). Asterisk indicates significant difference compared with WT in “mean difference two sided test” at α = 0.05. (C) Fragment of selected gel with stained protein bands localized between 15 and 10 kDa and “Image Lab 6.01” analyses of the intensity of these bonds.