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. 2021 Mar 16;22(6):3006. doi: 10.3390/ijms22063006

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Ultrastructure of mesophyll cells of wild type (A–C) and lpeat1 lpeat2 double mutant (D,E) of A. thaliana. (A) In vacuole there are numerous membrane structure i.e.,: multivesicular structures (MVS), multilamellar structure (MLS), and single-membrane-bounded vesicles (arrowheads); (B) Chloroplast in vacuole (arrowheads) with disturbed thylakoids and concentric membranes (arrowheads); (C)The MVS with bigger and less regularly shaped vesicles and MLS with less packed membranes in comparison to similar structures on A; (D,E) Single-membrane-bounded vesicles (arrowheads) in the vacuoles of the lpeat1 lpeat2 double mutant; (D) In the upper cell a structure reassembling Rubisco-containing bodies (RCBs) (arrow). Ch—chloroplast, V—vacuole, star—starch; Scale bar, 2 μm.