Figure 6.
Effect of DMG-gold on liver mitochondrial function. Mitochondrial respiration in the presence of succinate and rotenone (A) or palmitoyl-carnitine and malate (B) as substrates was determined in the presence (state 3) or absence (state 4) of adenosine diphosphate (ADP). (C) Carnitine palmitoyl transferase (CPT) activity in mitochondria is shown. (D) Ratio of pAMPK/AMPK from total liver extract of all animal groups, with representative immunoblots. The values of treated groups are expressed as percentage of the CTR group. (E) Hydrogen peroxide (H2O2) release and (F) superoxide dismutase (SOD) activity were determined in hepatic isolated mitochondria. Data are presented as means ± SEM from n = 7 animals/group, except for panel D (n = 5). CTR: control, HFD: high-fat diet, DMG: DMG-gold. Different superscripted letters on the histograms (a,b,c) indicate significantly different average values, p < 0.05.