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Generation of HLA-A allele-targeting scFvs and isogenic cell line models. (A) A2 scFv and (B) A3 scFv binding to various immobilized HLA alleles was assessed by ELISA. Data represent means ± SD of three technical replicates. (C) Flow cytometric evaluation with α-A2 (BB7.2-PE) and α-A3 (GAP.A3-APC) antibodies of HLA KO isogenic cancer cell lines following CRISPR-mediated HLA-A locus disruption.
