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. 2021 Mar 17;118(12):e2022410118. doi: 10.1073/pnas.2022410118

Fig. 3.

Fig. 3.

Determination of NASCAR specificity in vitro. Three isogenic cancer cell line models of LOH were employed to determine NASCAR specificity. Cancer cells with the indicated HLA-A allele status were coincubated with CAR or NASCAR T cells configured with the indicated allele-targeting CAR and iCAR receptor combinations at an E:T ratio of 2:1. T cell activation was assessed by ELISA for (A) human IFN-γ, hIFN-γ and (B) human IL-2, hIL-2 release. Data represent means ± SD of three technical replicates. (C) Cytotoxicity mediated by CAR or NASCAR T cells was measured by CellTiter-Glo (CFPAC-1, NCI-H441) or Steady-Glo (RPMI-6666). Data represent means ± SD of three technical replicates.