Fig. 3.

Regulation of HmpF by the Ptx and Pix systems. (A) Localization of HmpF-GFP in response to light in strains harboring deletions in genes encoding Ptx and Pix system components. Depicted are fluorescence micrographs of cellular autofluorescence (red) and GFP fluorescence (cyan) at each interval of a light regiment with exposure to white light for 1 min (L), followed by darkness for 1 min (1m D), and subsequently by white light for 1 min (1m L). (B and C) Quantification of the fraction of HmpF-GFP localized to the poles (B) and HmpF-GFP polar reversals (C) in various Ptx and Pix deletion strains. Error bars = ±1 SD; *P value < 0.05 as determined by two-tailed Student’s t-test between the wild type and each deletion strain; n = 3. B is derived from data depicted in SI Appendix, Fig. S6.