Fig. 4.

Effect of CCCP treatment on HmpF-GFP localization. (A) Localization of HmpF-GFP in response to treatment with CCCP or DMSO alone (– control) in a wild-type genetic background or strains harboring deletions in genes encoding Hmp system components. Depicted are fluorescence micrographs of cellular autofluorescence (red) and GFP fluorescence (cyan). (B) Quantification of the fraction of HmpF-GFP localized to the poles and cytoplasm. *P value < 0.05 as determined by two-tailed Student’s t-test between the CCCP and DMSO treatment for each strain and position measured; n = 3.